IgG2a-producing variants of an IgG2b-producing mouse myeloma cell line.
نویسندگان
چکیده
منابع مشابه
IgG2a-producing variants of an IgG2b-producing mouse myeloma cell line
12 variant cell lines producing an IgG2a (kappa) immunoglobulin derived via different routes from the IgG2b (kappa) synthesizing MPC 11 were studied. These variants all have the parental MPC 11 idiotype as shown by a radioimmunoassay. A comparison of the variants by charge, peptide maps, and assembly patterns has shown that most of them differ from one another, and some can be grouped. One grou...
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Cells of the MPC-11 mouse myeloma cell line, which produces an IgG2b immunoglobulin, were subjected to mutagenesis with Melphalan or with ICR-191, after which they were cloned in soft agar. Approximately 0.5 percent of the clones produced altered heavy chains that: (i) were the same size as or larger than the parent; (ii) no longer recognized by antibody specific for the parent gamma2b subclass...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1978
ISSN: 0022-1007,1540-9538
DOI: 10.1084/jem.147.6.1535